Manipulating Biology: Costs, Benefits and Controversies

CONTENTS OF CURRICULUM UNIT 18.05.01

  1. Unit Guide
  1. Introduction and Rationale
  2. Demographics
  3. Standards
  4. The Curriculum Unit
  5. Classroom Activities
  6. References

Augmenting Bone Regeneration: Structure, Function, and Dysfunction of the Skeletal System

Kwame Adu-Wusu

Published September 2018

Tools for this Unit:

References

Cong, L., & Zhang, F. (2015). Genome Engineering Using CRISPR-Cas9 System. Methods in Molecular Biology, 1239, 197-217.

Cribbs, A., & Perera, S. (2017). Science and Bioethics of CRISPR-Cas9 Gene Editing: An Analysis Towards Separating Facts and Fiction. YALE JOURNAL OF BIOLOGY AND MEDICINE, 90, 625-634.

Engineering T-Cells to Fight Cancer | Parker Institute for Cancer Immunotherapy. (2017). Retrieved from Parker Institute for Cancer Immunotherapy: https://www.parkerici.org/research_focus/best-in-class-t-cells/#progress

Friedlaender, G. E., Perry, C. R., Cierny, G., Muschler, G. F., Zych, G. A., Calhoun, J. A., . . . Yin, S. (2001). Osteogenic protein-1 (bone morphogenetic protein-7) in the treatment of tibial nonunions. The Journal of Bone and Joint Surgery, 83-A(Suppl 1(Pt 2)), 151-158.

Greenwald, A., Boden, S., Goldberg, V., Khan, Y., Laurencin, C., & Rosier, R. (2001). Bone-Graft Substitutes: Facts, Fictions, and Applications. THE JOURNAL OF BONE AND JOINT SURGERY, INCORPORATED, 83-A, 98-103.

International Osteoporosis Foundation. (2017). Retrieved from Facts and Statistics: https://www.iofbonehealth.org/facts-statistics

Kesselheim, A., & Avorn, J. (2016). Approving a Problematic Muscular Dystrophy Drug. JAMA.

Knothe Tate, M., Adamson, J., Tami, A., & Bauer, T. (2004). The osteocyte. The International Journal of Biochemistry & Cell Biology, 36, 1-8.

Kosicki, M., Tomberg, K., & Bradley, A. (2018). Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements. Nature Biotechnology, 1-8.

Lo, K.-H., Ashe, K., Kan, H., & Laurencin, C. (2012). The role of small molecules in musculoskeletal regeneration. Regen. Med, 7(4), 535-549.

Lu, C.-H., Chang, Y.-H., Lin, S.-Y., Li, K.-C., & Hu, Y.-C. (2013). Recent progresses in gene delivery-based bone tissue engineering. Biotechnology Advances, 31, 1695-1706.

Nauth, A., Ristevski, B., Li, R., & Schemitsch, E. (2011). Growth factors and bone regeneration: How much bone can we expect? Injury, 42, 574-579.

R.T. Franceschi. (2005). Biological Approaches to Bone Regeneration by Gene Therapy. Journal of Dental Research, 84(12), 1093-1103.

Reardon, S. (2016, 6 22). Nature. Nature News. doi:10.1038/nature.2016.20137

Roberts, T., & Rosenbaum, A. (2012). Bone grafts, bone substitutes and orthobiologics. Organogenesis, 8(4), 114-124.

Rosa, A. L. (2017). Research Supported by FAPESP. Gene therapy for bone regeneration using CRISPR-Cas9 system.

Southwood, L., Acvs, D., Frisbie, D., Kawcak, C., & Wayne Mcilwraith, C. (2004). Delivery of Growth Factors Using Gene Therapy to Enhance Bone Healing. Veterinary Surgery, 33, 565-578.

The Burden of the Musculoskeletal Diseases in the United States: Prevalence, Societal, and Economic Cost (3rd Edition). (2014). Retrieved from www.boneandjointburden.org

Vo, T., Kasper, F., & Mikos, A. (2012). Strategies for controlled delivery of growth factors and cells for bone regeneration ☆. Advanced Drug Delivery Reviews, 64, 1292-1309.

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