Genetic Engineering and Human Health

CONTENTS OF CURRICULUM UNIT 13.06.07

  1. Unit Guide
  1. Introduction & Background
  2. Rationale
  3. DNA Biology
  4. DNA in Forensic Science
  5. Polymerase Chain Reaction
  6. Gel Electrophoresis
  7. Strategies and Classroom Activities
  8. Bibliography
  9. Teacher Resources Bibliography
  10. Appendix 1
  11. Appendix 2
  12. Appendix 3
  13. Appendix 4
  14. Appendix 5
  15. Notes

DNA in Forensic Science: genetic engineering applications in forensics

Vanessa Vitug

Published September 2013

Tools for this Unit:

Appendix 5

Lab Activity – Micropipette Activity, By M. Okuda, modified by V. Vitug

Purpose: Practice using a 2-20ul micropipette

Materials – 4 dye colors 100ul in each vial, 1 (2-20ul) micropipette, wells (gridded stock paper)

Procedures/Protocols:

1. Obtain materials

2. Each student completes a row; note row, column & dye color;

3. Pipette only 7 ul per "well"

4. Read the hidden message and record

image 13.06.07.01

Message Reads:

# 6 card message reads: ____________

Conclusion: (Answer in a paragraph below)

Were you able to read the message? Where all the spots even in size and general shape, if not why? Describe the correct technique for pipetting.

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